- Title
- Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans
- Creator
- Shihana, Fathima; Wong, Wilson K. M.; Joglekar, Mugdha V.; Mohamed, Fahim; Gawarammana, Indika B.; Isbister, Geoffrey K.; Hardikar, Anandwardhan; Seth, Devanshi; Buckley, Nicholas A.
- Relation
- NHMRC.ID1055176 http://purl.org/au-research/grants/nhmrc/1055176
- Relation
- Scientific Reports Vol. 11, Issue 1, no. 9165
- Publisher Link
- http://dx.doi.org/10.1038/s41598-021-87918-0
- Publisher
- Nature
- Resource Type
- journal article
- Date
- 2021
- Description
- RNAs in biofluids are potential biomarkers for detecting kidney and other organ injuries. We profiled microRNAs in urine samples from patients with Russell’s viper envenoming or acute self-poisoning following paraquat, glyphosate, or oxalic acid [with and without acute kidney injury (AKI)] and on healthy controls. Discovery analysis profiled for 754 microRNAs using TaqMan OpenArray qPCR with three patients per group (12 samples in each toxic agent). From these, 53 microRNAs were selected and validated in a larger cohort of patients (Russell’s viper envenoming = 53, paraquat = 51, glyphosate = 51, oxalic acid = 40) and 27 healthy controls. Urinary microRNAs had significantly higher expression in patients poisoned/envenomed by different nephrotoxic agents in both discovery and validation cohorts. Seven microRNAs discriminated severe AKI patients from no AKI for all four nephrotoxic agents. Four microRNAs (miR-30a-3p, miR-30a-5p, miR-92a, and miR-204) had > 17 fold change (p < 0.0001) and receiver operator characteristics area-under-curve (ROC-AUC) > 0.72. Pathway analysis of target mRNAs of these differentially expressed microRNAs showed association with the regulation of different nephrotoxic signaling pathways. In conclusion, human urinary microRNAs could identify toxic AKI early after acute injury. These urinary microRNAs have potential clinical application as early non-invasive diagnostic AKI biomarkers.
- Subject
- acute kidney injury; diagnostic markers; miRNAs; toxicology
- Identifier
- http://hdl.handle.net/1959.13/1449408
- Identifier
- uon:43656
- Identifier
- ISSN:2045-2322
- Rights
- © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
- Language
- eng
- Full Text
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